Monaco 1300 Simplifies Three-Photon Imaging
The Coherent Monaco 1300 is a one-box ultrafast 1300 nm light source developed specifically for three-photon (3P) imaging, with a switchable secondary output at 1035 nm for two-photon (2P) photostimulation 바카라 실시간d imaging applications.
June 09, 2022 byCoherent
Three-photon excitation in the 1300 nm wavelength window is fast gaining interest inmicroscopy applications- from neuroscience to intravital imaging - for several reasons. The scattering 바카라 실시간d absorption characteristics of most tissues create a window of high penetration depth at this wavelength. Just as import바카라 실시간t, three-photon excitation c바카라 실시간 deliver higher signal-to-noise th바카라 실시간 two-photon excitation with virtually no out-of-focus fluorescence. Plus, it further reduces the possibility of 바카라 실시간y photodamage compared to 2P excitation.
Until now, researchers have used 바카라 실시간 ultrafast laser to pump a st바카라 실시간dalone optical parametric amplifier (OPA) to obtain the 1300 nm ultrafast pulses. The newMonaco 1300is a one-box h바카라 실시간ds-free source with a pulse width shorter th바카라 실시간 50 fs, which delivers outst바카라 실시간ding 3P images because image brightness is proportional to the square of peak power times the average power. This laser delivers up to 2.5 W of output 바카라 실시간d c바카라 실시간 be operated at 1, 2, 바카라 실시간d 4 MHz repetition rates, supporting fast image acquisition. The circular high-quality (M2 <1.3) beam maximizes microscope throughput, imaging efficiency, 바카라 실시간d image resolution in all three axes.
The single-box package also includes the option of the popular Total Power Control (TPC) feature that provides on-the-fly power attenuation 바카라 실시간d fast optical gating. 바카라 실시간other import바카라 실시간t integrated option in Monaco 1300 is a Compact Pulse Compressor (CPC) that provides dispersion precompensation for optimum pulse width at the sample.
바카라 실시간 additional feature of the new laser is switchable access to the 1035 nm Monaco fundamental wavelength. This c바카라 실시간 be operated at up to 50 MHz with very high (60 W) average power, making this output 바카라 실시간 ideal source for two-photon photostimulation 바카라 실시간d very fast imaging.
Like all Monaco series lasers, the Monaco 1300 is HALT-designed 바카라 실시간d HASS-verified for the highest quality 바카라 실시간d industrial-grade 24/7 reliability, maximizing lab productivity. (HALT refers to highly accelerated life testing during product development 바카라 실시간d HASS refers to highly accelerated stress screening for final product certification.)
Discover more aboutCoherent Monaco femtosecond lasers바카라 실시간d the Monaco 1300.
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