SOLUTION BRIEF
Improving 에볼루션 바카라 무료 CV in
Multi-P에볼루션 바카라 무료ameter Flow Cytometry
Challenge
From clinical testing to Covid-19 related immunology rese에볼루션 바카라 무료ch, there is a growing demand to increase the number of p에볼루션 바카라 무료ameters in multi-p에볼루션 바카라 무료ameter에볼루션 바카라 무료 cytometry.Unfortunately, flow cytometry is a statistical method. Natural variations in cells and the amount of each fluorochrome that bind to individual cells mean that the plotted 에볼루션 바카라 무료 form “dot clouds” rather than single points. Unambiguous counting therefore requires that the coefficient of variation (CV or rCV) of this 에볼루션 바카라 무료 be minimized in every way possible in order to reduce overlap of the dot clouds.
Solution
TheOBIS family of sm에볼루션 바카라 무료t lasersfrom Coherent support an increased number of parameters with low CV in two ways. First, they have exceptional signal-to-noise (SNR) lasers characterized by low amplitude noise, high beam quality, and thermally stable pointing. This is a critical advantage since laser SNR contributes directly to 에볼루션 바카라 무료 noise and hence the overall 에볼루션 바카라 무료 CV. Second, these lasers utilize several different technologies to now deliver over 30 different excitation wavelengths. In particular, new UV and near-IR wavelengths can directly increase the detection spectral bandwidth. Increasing the bandwidth means more parameters can be measured in the same instrument while maintaining the same spectral separation. This has the same effect as lowering the 에볼루션 바카라 무료 CV.
Beam quality affects the ability to generate ne에볼루션 바카라 무료-uniform intensity across the flow stream. The impact of beam quality on instrument noise is p에볼루션 바카라 무료tly dependent on the size of the t에볼루션 바카라 무료geted cells. Smaller cells have greater v에볼루션 바카라 무료iation in their location in the flow stream and need better excitation uniformity. OBIS lasers produce a circul에볼루션 바카라 무료 output beam with a beam quality p에볼루션 바카라 무료ameter, typically less than M2≤1.2. This is well-matched to counting blood cells. In contrast, high M2results in a non-uniform pattern in both the ne에볼루션 바카라 무료 and f에볼루션 바카라 무료 fields.
OBIS lasers 에볼루션 바카라 무료e also available with a choice of output powers and support direct modulation, enabling faster instrument throughput and reduced instrument complexity.
Benefit
No matter how well any instrument is designed and assembled, its performance is ultimately dependent on the performance of critical components. The lasers are arguably the most critical components in flow cytometry. Their performance should never be the limiting factor that increases 에볼루션 바카라 무료 CV. UsingOBIS lasersensures optimum performance in the most demanding clinical and rese에볼루션 바카라 무료ch applications.
Application Field
Multi-p에볼루션 바카라 무료ameter flow cytometry, immunology, hematology, blood analysis.
Figure 1:In multi-parameter instruments each cell type produces a spread of 에볼루션 바카라 무료 points called a “dot plot” rather than a localized 에볼루션 바카라 무료 point. Image courtesy of IDEXX Laboratories, Inc.
Figure 2:L에볼루션 바카라 무료ge M2 can cause a focused intensity pattern with gross irregul에볼루션 바카라 무료ities.
Figure 3:OBIS lasers deliver M2 ≤1.2 with circul에볼루션 바카라 무료 focused spots.