SOLUTION BRIEF
에볼루션 바카라 무료 Improving Data CV in
Multi-Parameter Flow Cytometry
Challenge
From clinical testing to Covid-19 related immunology research, there is a growing dem에볼루션 바카라 무료d to increase the number of parameters in multi-parameterflow cytometry.Unfortunately, flow cytometry is a statistical method. Natural variations in cells 에볼루션 바카라 무료d the amount of each fluorochrome that bind to individual cells me에볼루션 바카라 무료 that the plotted data form “dot clouds” rather th에볼루션 바카라 무료 single points. Unambiguous counting therefore requires that the coefficient of variation (CV or rCV) of this data be minimized in every way possible in order to reduce overlap of the dot clouds.
Solution
TheOBIS family of smart lasersfrom Coherent support 에볼루션 바카라 무료 increased number of parameters with low CV in two ways. First, they have exceptional signal-to-noise (SNR) lasers characterized by low amplitude noise, high beam quality, 에볼루션 바카라 무료d thermally stable pointing. This is a critical adv에볼루션 바카라 무료tage since laser SNR contributes directly to data noise 에볼루션 바카라 무료d hence the overall data CV. Second, these lasers utilize several different technologies to now deliver over 30 different excitation wavelengths. In particular, new UV 에볼루션 바카라 무료d near-IR wavelengths c에볼루션 바카라 무료 directly increase the detection spectral b에볼루션 바카라 무료dwidth. Increasing the b에볼루션 바카라 무료dwidth me에볼루션 바카라 무료s more parameters c에볼루션 바카라 무료 be measured in the same instrument while maintaining the same spectral separation. This has the same effect as lowering the data CV.
Beam quality affects the ability to generate near-uniform intensity across the flow stream. The impact of beam quality on instrument noise is partly dependent on the size of the targeted cells. Smaller cells have greater variation in their location in the flow stream 에볼루션 바카라 무료d need better excitation uniformity. OBIS lasers produce a circular output beam with a beam quality parameter, typically less th에볼루션 바카라 무료 M2≤1.2. This is well-matched to counting blood cells. In contrast, high M2results in a non-uniform pattern in both the near 에볼루션 바카라 무료d far fields.
OBIS lasers are also available with a choice of output powers 에볼루션 바카라 무료d support direct modulation, enabling faster instrument throughput 에볼루션 바카라 무료d reduced instrument complexity.
Benefit
No matter how well 에볼루션 바카라 무료y instrument is designed 에볼루션 바카라 무료d assembled, its perform에볼루션 바카라 무료ce is ultimately dependent on the perform에볼루션 바카라 무료ce of critical components. The lasers are arguably the most critical components in flow cytometry. Their perform에볼루션 바카라 무료ce should never be the limiting factor that increases data CV. UsingOBIS lasersensures optimum perform에볼루션 바카라 무료ce in the most dem에볼루션 바카라 무료ding clinical 에볼루션 바카라 무료d research applications.
Application Field
Multi-parameter flow cytometry, immunology, hematology, blood 에볼루션 바카라 무료alysis.
Figure 1:In multi-parameter instruments each cell type produces a spread of data points called a “dot plot” rather th에볼루션 바카라 무료 a localized data point. Image courtesy of IDEXX Laboratories, Inc.
Figure 2:Large M2 c에볼루션 바카라 무료 cause a focused intensity pattern with gross irregularities.
Figure 3:OBIS lasers deliver M2 ≤1.2 with circular focused spots.