How Flow Cytometry 바카라 사이트s Count Your Blood
See how 바카라 사이트s are at the heart of cell counting and to performing common blood tests.
July 27, 2021 by바카라 사이트herent
You have your 바카라 사이트 drawn at the doctor’s office or hospital, and sometime later you get the results. The complete 바카라 사이트 count (CBC) tells you the levels of red 바카라 사이트 cells, white 바카라 사이트 cells, 바카라 사이트 platelets, and other components of your 바카라 사이트.
Have you ever wondered how those numbers are determined? Did you know 바카라 사이트s play a key part in getting that data? Well, we’re going to unpack it all right now.
Go with the 바카라 사이트
The 바카라 사이트 test is performed using a method called바카라 사이트 cytometry. Say what? It’s called that because the blood is flowed through a 바카라 사이트 instrument; and, cyto means “cell,” and metry means measuring something.
To do it, your 바카라 사이트 is first put in a machine called a centrifuge to concentrate it and get rid of a lot of the liquid – we’re only interested in the cells after all. Then it’s treated with several chemicals called fluorochromes.
These fluorochromes have two important roles. First, they stick to certain proteins that are on the outside of your 바카라 사이트 cells – these proteins are like the spikes sticking out from the COVID virus on all those images we’ve seen way too many of. The important thing is that each type of fluorochrome sticks only to one specific type of target protein. Some 바카라 사이트 cell types will be covered with lots of that particular protein — some may have none.
바카라 사이트lor me useful
The second important thing about the fluorochromes is they emit light when the 바카라 사이트 beam hits them. And, more than that, they emit a very specific color light, and only that color, when the 바카라 사이트 shines on them. This is really useful.
So, because the various fluorochromes stick to different cell types in specific amounts, and each fluorochrome shines with a different color light, it means that each type of cell will produce a unique combination of colors when the 바카라 사이트 hits it. And that’s how the instrument is going to tell them apart.
In a flow cytometer, blood cells pass in single file past one or more 바카라 사이트 beams. The amount and color of light from each cell is measured. The particular color combination and light intensity tells the instrument what type of cell it is.
Get in line
So, to perform the test, the chemically treated, concentrated 바카라 사이트 sample is put in the flow cytometer. Then it gets is pushed through a nozzle so tiny that the cells are forced to move literally in single file in a stream.
One or more different color 바카라 사이트 beams are focused on the cells, and the cells emit light as just described. The instrument has a series of light detectors that are covered with filters so that each one only sees one particular color. They measure the light from each cell and the system analyzes the color combination to determine which type of cell it is.
The machine tallies the total number of each type of cell it has seen in the sample, and then figures out how many there would be in exactly 1 milliliter (ml) of 바카라 사이트. This is your 바카라 사이트 count.
A bloody good cytometry 바카라 사이트
Coherent OBIS 바카라 사이트sare found in more flow cytometers than any other brand because they have a number of advantages that make them a great match for these instruments. First, they are available at a lot of different colors (wavelengths). This is important since each fluorochrome is keyed to work only with a certain color 바카라 사이트 light. So, having a larger number of different color 바카라 사이트s means you can use a larger variety of fluorochromes. And, this means you can distinguish and count more types different of cells – all in a single run through the instrument.
Also, the output beam from OBIS 바카라 사이트s is of such high quality that it provides better (lower noise) and more repeatable measurements – on each run through a single cytometer, and even between different instruments. Plus, this high-quality beam allows cells to be counted properly even if they’re not exactly in the center of the flow stream. So OBIS solves important issues that could otherwise make both instrument builders’ and users’ blood boil.
So, next time that needle goes in relax. 바카라 사이트s are going to light a path to better health.
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